Forensic Applications of Whole Genome Amplification
نویسنده
چکیده
Whole genome amplification (WGA) has been touted as a possible technique to augment fragment analysis of STRs in amplifying low-levels of DNA recovered from crime scenes or items. Only LCN DNA analysis is routinely used as an additional amplification step; but this is not without problems, such as the increased incidents of stochastic variations. A review of the literature was carried out in order to assess the latest research and to identify a potentially fit for purpose whole genome amplification technique. There are three potential alternative techniques which show promise, miPEP, dcDOP-PCR and MDA used in conjunction with a macromolecular crowder; with the majority of studies investigating the effect of non-crowded MDA on a variety of stains, including degraded, low-level and stains containing
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